What is Reverse complementary?
- In a DNA molecule, the two antiparallel strands (positive or 5’-> 3’ and negative or 3’-> 5’) are complementary to each other
- As per standard notation, we write the DNA sequence in 5’-> 3’ orientation. Therefore, to represent a sequence of the negative strand in 5’-> 3’ orientation we need to perform a reverse complementary of positive strand (e.g. reverse or left primer)
How to obtain reverse complementary of DNA sequence?
- We will use
- Check bioinfokit documentation for installation and documentation
- Download test data dataset
# you can use interactive python interpreter, jupyter notebook, spyder or python code # I am using interactive python interpreter (Python 3.7) # find reverse complemenatry of a given DNA sequence >>> from bioinfokit import analys >>> analys.rev_com(seq="AGTCAGTAACTAG") # output: CTAGTTACTGACT # if you have sequences in a fasta file >>> analys.rev_com(file="test.fa") # output will be saved in same directory (output_revcom.fasta)
This work is licensed under a Creative Commons Attribution 4.0 International License