Reverse complementary

Renesh Bedre less than 1 minute read

What is Reverse complementary?

In a DNA molecule, the two antiparallel strands (positive or 5’-> 3’ and negative or 3’-> 5’) are complementary to each other
As per standard notation, we write the DNA sequence in 5’-> 3’ orientation. Therefore, to represent a sequence of the negative strand in 5’-> 3’ orientation we need to perform a reverse complementary of positive strand (e.g. reverse or left primer)

How to obtain reverse complementary of DNA sequence?

We will use bioinfokit
Check bioinfokit documentation for installation and documentation
Download test data dataset

# you can use interactive python interpreter, jupyter notebook, spyder or python code
# I am using interactive python interpreter (Python 3.7)
# find reverse complemenatry of a given DNA sequence
>>> from bioinfokit import analys
>>> analys.rev_com(seq="AGTCAGTAACTAG")
# output: CTAGTTACTGACT

# if you have sequences in a fasta file
>>> analys.rev_com(file="test.fa")
# output will be saved in same directory (output_revcom.fasta)